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Image Search Results
Journal: Neuron
Article Title: CD49f is a novel marker of functional and reactive human iPSC-derived astrocytes
doi: 10.1016/j.neuron.2020.05.014
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Mouse neurons were treated with 70% ACM with 5μM IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius; 4440) and imaged every 6 hours for 60 hours on an
Techniques: Purification, Produced, Recombinant, Marker, Glutamate Assay, Imaging, Apoptosis Assay, Labeling, RNA Sequencing Assay, Transgenic Assay, Expressing, Software, Luminex, Western Blot
Journal: World Journal of Gastroenterology
Article Title: Deciphering lactate metabolism in colorectal cancer: Prognostic modeling, immune infiltration, and gene mutation insights
doi: 10.3748/wjg.v31.i25.107478
Figure Lengend Snippet: Prognostic modeling of lactate metabolism characteristics in colorectal cancer. A: Screening for the optimal penalty parameter (λ) in LASSO Cox analysis; B: Coefficients of genes in LASSO Cox analysis; C: Forest plot of seven independent prognostic factors; D: Heatmap of the expression levels of 7 lactate metabolism genes in the high- and low-risk score groups; E: Risk score distribution of samples in The Cancer Genome Atlas database-colon and rectal adenocarcinomas (TCGA-COADREAD) cohort; F: Scatterplot of the survival status of COADREAD patients in the TCGA-COADREAD cohort according to the risk score distribution; G: Principal component analysis distribution plot of patients with high and low risk scores based on the expression profiles of 7 genes related to lactate metabolism; H: Kaplan-Meier survival curves of patients in the TCGA-COADREAD cohort with high and low risk scores; I: Receiver operating characteristic curves of patients in the TCGA-COADREAD cohort with the risk score for predicting 1-, 3-, and 5-year survival; J: Receiver operating characteristic curves for the risk score for predicting patient survival at 1, 3, and 5 years in the GSE103479 cohort. a P < 0.05, c P < 0.001. MT-ND2: Mitochondrial-dihydronicotinamide adenine dinucleotide dehydrogenase subunit 2; RBM3: RNA binding motif protein 3; ACADVL: Acyl-CoA dehydrogenase very-long-chain; SLC2A3: Solute carrier family 2 member 3; BATF: Basic leucine zipper transcription factor ATF-like; ICOS: Inducible T cell costimulator; LEPROTL1: Leptin receptor overlapping transcript-like 1; PCA: Principal component analysis; ROC: Receiver operating characteristic; AUC: Area under the curve; TCGA: The Cancer Genome Atlas database.
Article Snippet: Primary antibodies were: Anti-leptin receptor overlapping transcript-like 1 (anti-LEPROTL1) antibody (TA331958, Origene, MD, United States), anti-inducible T cell costimulator (anti-ICOS) antibody (AP13108PU-N, Origene, MD, United States), anti-RNA-binding protein 3 antibody (EPR6061, Abcam, United Kingdom), anti-basic leucine zipper transcription factor ATF-like (BATF) antibody (EPR21911-55, Abcam, United Kingdom), anti-solute carrier family 2 member 3 (anti-SLC2A3) antibody (LS-B7024-50, Lifespan Biosciences, WA, United States), anti-acyl-CoA dehydrogenase
Techniques: Expressing, RNA Binding Assay
Journal: World Journal of Gastroenterology
Article Title: Deciphering lactate metabolism in colorectal cancer: Prognostic modeling, immune infiltration, and gene mutation insights
doi: 10.3748/wjg.v31.i25.107478
Figure Lengend Snippet: Expression levels of biomarkers in colorectal cancer. A: Immunohistochemistry results demonstrating the expression level of histone H3 lysine 18 lactylation in colorectal cancer (CRC) tissues and paracancerous tissues; B: Immunofluorescence results of histone H3 lysine 18 lactylation levels in tumor tissues and paracancerous tissues; C: Western blot results demonstrating the protein expression levels of mitochondrial-dihydronicotinamide adenine dinucleotide dehydrogenase subunit 2 (MT-ND2), acyl-CoA dehydrogenase very-long-chain (ACADVL), solute carrier family 2 member 3 (SLC2A3), basic leucine zipper transcription factor ATF-like (BATF), RNA binding motif protein 3 (RBM3), inducible T cell costimulatory (ICOS), leptin receptor overlapping transcript-like 1 (LEPROTL1) in CRC tissues/paracancerous tissues, and normal colon cell/CRC cell lines (SW620, HCT116, WS480); D: The quantitative real-time fluorescence polymerase chain reaction results demonstrating the expression levels of MT-ND2, ACADVL, SLC2A3, BATF, RBM3, ICOS, and LEPROTL1 in CRC tissues and paracancerous tissues; E: The quantitative real-time fluorescence polymerase chain reaction results demonstrate the expression levels of MT-ND2, ACADVL, SLC2A3, BATF, RBM3, ICOS, and LEPROTL1 in normal colon cell and CRC cell lines (SW620, HCT116, WS480). c P < 0.001, compared with normal group. H3K18 La: Histone H3 lysine 18 lactylation; MT-ND2: Mitochondrial-dihydronicotinamide adenine dinucleotide dehydrogenase subunit 2; ACADVL: Acyl-CoA dehydrogenase very-long-chain; SLC2A3: Solute carrier family 2 member 3; BATF: Basic leucine zipper transcription factor ATF-like; RBM3: RNA binding motif protein 3; ICOS: Inducible T cell costimulatory; LEPROTL1: Leptin receptor overlapping transcript-like 1; FHC: Normal colon cells.
Article Snippet: Primary antibodies were: Anti-leptin receptor overlapping transcript-like 1 (anti-LEPROTL1) antibody (TA331958, Origene, MD, United States), anti-inducible T cell costimulator (anti-ICOS) antibody (AP13108PU-N, Origene, MD, United States), anti-RNA-binding protein 3 antibody (EPR6061, Abcam, United Kingdom), anti-basic leucine zipper transcription factor ATF-like (BATF) antibody (EPR21911-55, Abcam, United Kingdom), anti-solute carrier family 2 member 3 (anti-SLC2A3) antibody (LS-B7024-50, Lifespan Biosciences, WA, United States), anti-acyl-CoA dehydrogenase
Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Western Blot, RNA Binding Assay, Fluorescence, Polymerase Chain Reaction